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The soluble fraction (supernatant) was separated from the membrane fraction, which was washed twice with 50 mM TRIS-HCl, pH 7.0 and centrifuged (150,000 × g , 4 °C, 120 min)

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Tofacitinib also significantly inhibits IL-22-induced CXCL1 and CXCL5 expression in human colonic tissues, suggesting that targeting the IL-22/CXC chemokine axis through JAK/STAT inhibition may be a viable therapeutic strategy